Selection of a potent inhibitor of trihydroxynaphthalene reductase by sorting disease control data

Bioorg Med Chem Lett. 2000 Mar 6;10(5):491-4. doi: 10.1016/s0960-894x(00)00037-8.

Abstract

Compounds that control rice blast, but not other crop diseases, were selected for testing as inhibitors of trihydroxynaphthalene reductase of the fungal melanin biosynthetic pathway. A potent inhibitor of the enzyme (2) (Ki = 25 nM) was identified. An X-ray structure of the enzyme-NADPH-2 complex was determined at 2.1 A resolution.

MeSH terms

  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Databases, Factual
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology*
  • Fungal Proteins*
  • Fungi / drug effects*
  • Melanins / biosynthesis
  • Molecular Conformation
  • NADP / chemistry
  • Oryza
  • Oxidoreductases / antagonists & inhibitors*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Plant Diseases / microbiology*
  • X-Ray Diffraction

Substances

  • Antifungal Agents
  • Enzyme Inhibitors
  • Fungal Proteins
  • Melanins
  • NADP
  • Oxidoreductases
  • tetrahydroxynaphthalene reductase
  • Oxidoreductases Acting on CH-CH Group Donors